Background: Gastritis is an invariable finding in patients infected with Helicobacter pylori (H. pylori).
Genistein is the predominant isoflavone in soybean, a specific inhibitor of tyrosine specific-protein kinase that has
been demonstrated an anti-inflammatory property. The aim of this study was to determine the effects of genistein
on H. pylori-associated gastritis in a rat model.
Methods: Male Sprague-Dawley rats were randomLy divided into three groups: group 1 (Control group),
group 2 (H. pylori infection group), and group 3 (Genistein treatment group). The control group was treated with
0.1% DMSO (1 mL/rat, b.i.d.) for 17 day. In the H. pylori infection group and the genistein treatment group, pretreatment
with streptomycin suspended in drinking water (at dose 5 mg/mL) for 3 days, then the rats were inoculated
with H. pylori suspension (108-10 CFU/mL ; 1 mL/rat, b.i.d.) for 3 consecutive days. Thereafter, rats in the
genistein treatment group were treated with genistein (16 mg/kg BW b.i.d.) for 14 days. The rats were then sacrificed
at the end of the experimental protocol. Serum samples were collected for measurement of TNF-α level and
CINC-1 level. The stomach was removed for detection H. pylori infection by urease test and for pathological
examination by a pathologist.
Results: The levels of serum TNF-α and CINC-1 were significantly higher in the H. pylori infection
group than in the control group (43.50 ± 16.51 vs. 20.89 ± 8.90 pg/mL, 138.10 ± 43.56 vs. 81.27 ± 19.89 pg/mL,
p<0.05, respectively). As expected, the levels of serum TNF-α and CINC-1 were significantly lower in the genistein
group than in the H. pylori infection group (29.33 ± 10.77 vs. 43.50 ± 16.51 pg/mL, 103.26 ± 23.76 vs. 138.10 ±
43.56 pg./mL, p<0.05, respectively). In H. pylori infection group, almost all of the stomach tissues showed moderate
gastric inflammation and moderate to mark H. pylori colonization score. In genistein treatment group, stomach
histopathology was improved when compared to H. pylori infection group, especially in the reduction of inflammatory
Conclusion: Administration of genistein can attenuate H. pylori-induced gastritis in rats, possibly by
reducing the inflammatory mediators and thereby improving gastric pathology.