Abstract :
Background: Acetaminophen (APAP) overdose result in hepatic damage via several mechanisms in-
cluding increase production of cytokines, oxidative stress and decrease hepatic glutathione (GSH) which play an
important role in detoxification. Aloe vera (A. vera) is a species of succulent plant. Extract of A. vera has been
reported in antioxidative, anti-inflammatory effect. This study aim to determine the protective effect and mecha-
nism of extract from A. vera on APAP-induced hepatotoxicity.
Methods: Twenty-four male mice (25-30 gram) were randomly divided into 3 groups; control, APAP and
A.vera-treated group. In control group mice were fed with distilled water, while mice in APAP group were fed with
APAP 400 mg/kg single dose and APAP 400 mg/kg plus A.vera 150 mg/kg single dose in A.vera-treated group. The
serum and hepatic tissue was collected to identify activities of aspartate aminotransferase (AST), alanine ami-
notransferase (ALT), hepatic GSH, hepatic malondialdehyde (MDA) which is marker of oxidative stress and histo-
pathology.
Results: In APAP group, serum AST, ALT and hepatic MDA were significant increased and hepatic
GSH were significant decreased when compared to control group. The levels of AST, ALT, hepatic GSH and MDA
in A.vera-treated group were obvious improved as compared with APAP group. In A.vera-treated group, the degree
of hepatic necro-inflammation was lower than in APAP group.
Conclusion: Aloe vera can prevent hepatic damage from acetaminophen overdose primarily by restor-
ing hepatic glutathione and decrease oxidative stress. |
file/Thai-Journal-of-gastroenterology-vol-14-no-1-1594346.pdf