Thai Journal of Gastroenterology

Article :
A Simple Rat Model of Chronic Helicobacter pylori Infection for Research Study

Author :
Duangporn Thong-Ngam, M.D.
Ratsamee Prabjone, M.D.
Naruemon Videsopas, M.D.
Thanita Chatsuwan, M.D.

Abstract :

Background: H. pylori is now become accepted as a human pathogen for the development of gastritis and gastro-duodenal ulcer diseases.

Objective: To develop a simple rat model of chronic H. pylori infection for research study in the future.
Materials and Methods: Eighty-five Spraque-Dawley rats were divided into three groups.  The first
group of 63 rats was pretreatment with streptomycin and then was inoculated with H. pylori.  The second group of 10 rats was pretreatment with omeprazole and then was inoculated with H. pylori.  The third group of 12 saline inoculated rats were served as a control group.  Two weeks after inoculation, rats were sacrificed and the stomachs were removed.  Antral biopsies were performed for urease test and the stomachs were taken for histopathology. The successful of H. pylori inoculation is defined as positive both urease test and histopathology.

Results: There were 44/63 (69.8%) in group 1 and 6/10 (60.0%) in group 2 success of H. pylori inocu-
lation, respectively.  Histopathology detected organism along mucous lining the surface epithelium and crypt lumen and demonstrated mild to moderate gastric inflammation in the successful inoculated rats. There were normal histopathology and no organism in the control group and group that failure of H. pylori inoculation.  The results of urease test and pathology are all concordance.

Conclusion: In this study, we purposed the simple model of chronic H. pylori infection in rat.  There was
a favorable successful rate and was accompanied by a mild to moderate mucosal inflammation.  This animal model could apply for research studies in the future.

[Thai J Gastroenterol 2005; 6(1): 3-7]

Keyword :
Helicobacter pylori, rat model, chronic infection

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