Abstract :
Background: Helicobacter pylori is a bacterium implicated in the etiology of gastritis and peptic ul-
cers. Certain herbal agents have been reported to have antibacterial activity against H. pylori.
Aim: To assess the in vitro anti-H. pylori potential of four herbal substances, namely Aloe vera, curcumin,
garlic, and plau-noi.
Methods and Materials: A standard strain (NCTC 11637) and 9 clinical isolates of H. pylori were
used to assess antibacterial activity of Aloe vera, curcumin, garlic, and plau-noi, employing an agar dilution and disk diffusion method. The agar dilution was tested by two-fold dilution of various concentrations of herbal substances, ranging 0.016-512 micrograms/mL on Columbia blood agar. The disk diffusion was tested using varying amounts of herbal substance at 20, 50, 100, 200 and 400 micrograms.
Results: The minimum inhibitory concentrations (MICs) of Aloe vera, curcumin, garlic, and plaunotol
were >512, 64, >512, >512 micrograms/mL, respectively. There were no inhibition zones found when testing with Aloe vera, garlic, and plau-noi, although the inhibition zone diameters of 6.5-8 mm and 10-12 mm were detected at 200 and 400 micrograms curcumin disk, respectively.
Conclusion: Curcumin exhibited potential of in vitro antibacterial activity against H. pylori, which
suggests that it may be useful for the treatment of H. pylori infection. Aloe vera, garlic, and plau-noi, on the other hand, did not show antibacterial activity against H. pylori in this study.
[Thai J Gastroenterol 2007; 8(1): 5-11] |
file/Thai-Journal-of-gastroenterology-vol-8-no-1-1618382.pdf