Abstract :
AIM: To determine the effects of Lactobacillus plantarum B7 (L. platarum B7) reduces pro-inflammatory
cytokines (TNF-α level, Interleukin-6level and CXCL1 level) and attenuates the physical symptoms of S.
Typhimurium induced diarrhea in mice.
METHODS: Male albino mice were randomly divided into 3 groups: control group (n=8), mice were
fed with 1 mL of 0.85% saline by oral gavage feeding. Salmonella group (n=8), mice were fed with 3x109 CFU of
S. Typhimurium 1 mL suspended in 0.85% saline, and Salmonella +LP group (n=8), mice were fed with 1x109 CFU
of L. plantarum B7 suspended in 0.85% saline. After 2 hours, mice were fed with 3x109 CFU of S. Typhimurium
suspended in 1 mL of 0.85% saline for 3 consecutive days. All groups received a 3-day pre-treatment with streptomycin
suspended in drinking water (5 mg/mL). The body weight of mice were measured and recorded daily. After
3 days, fresh specimens of feces were collected for stool culture and colony counts to assess S. Typhimurium
infection. Blood samples were also collected to determine TNF-α level, Interleukin-6 and CXCL1 levels. Fecal
characteristics and the percentage of fecal moisture content (%FMC) were measured.
RESULTS: The quantitative of S. Typhimuriumin in fecal specimens significantly decreased in the Salmonella
+ LP group compared with the Salmonella group (7.4.2±0.05 vs 8.86±0.02 CFU, p<0.05). The levels of
TNF-α, IL-6 and CXCL1 significantly increased in the Salmonella group compared with the control group
(128.59±12.82 vs. 53.49±8.90, 144.44±8.91 vs. 66.51±4.04, 96.09±10.81 vs. 32.32±4.54 pg/mL respectively, p<0.05)
and significantly decreased in the Salmonella+LP group compared with the Salmonella group (36.15±9.22 vs.
128.59±12.82, 70.36±5.37 vs. 144.44±8.91, 35.40±2.77 vs. 96.09±10.81 pg/mL respectively, p<0.05). Fecal consistency
was soft or loose in the Salmonella group, and was rod-shaped and dark in the Salmonella+LP group. Fecal
moisture percentage (%FMC) significantly increased in the Salmonella group compared with the control group
(43.24±2.05% vs. 14.19±1.57%, p<0.05), and significantly decreased in the Salmonella+LP group compared with
the Salmonella group (24.65±2.08% vs. 43.24±2.05%, p<0.05).
CONCLUSIONS: Oral administration of L.plantarum B7 can inhibit S. Typhimurium growth, decrease
pro-inflammatory cytokine levels, attenuate inflammatory response and improve fecal moisture. L.plantarum B7
can prevent S. Typhimurium diarrhea in mice.
|
file/Thai-Journal-of-gastroenterology-vol-16-no-2-402389.pdf