Abstract :
Background: Non-steroidal anti-inflammatory drugs (NSAIDs) are one of the most commonly prescribed
worldwide and known to induce gastric injury from multiple mechanisms. Aloe vera is known to effectively
decrease inflammation and promote ulcer healing but there are still limited data.
Objective: To investigate the effects of Aloe vera on NSAIDs-induced gastropathy and its effects on
serum TNF-α, IL-1β, CINC-1, and tissue MDA in rats.
Materials and Methods: Male Wistar rats were randomly divided into three groups (n=6, each). Group
1 (control group): was fed with sterile water. Group 2 (NSAIDs group): was fed with 150 mg/kg indomethacin
dissolved in 5% sodium bicarbonate at 0 h and 4 h. Group 3 (Aloe vera-treated group): was fed with 150 mg/kg
indomethacin as previously described and 200 mg/kg Aloe vera at 0 h and 4 h. The stomach was removed to study
gastric histopathology at 8 h after experiment. Serum and gastric tissue were collected to determine TNF-α and
CINC-1 using ELISA technique and gastric MDA using TBARS assay kit.
Results: In NSAIDs group, serum TNF-α, CINC-1and gastric MDA were significantly increased when
compared to control group (27.8±1.5 vs. 85.1±49.1 pg/mL, 104.5±45.8 vs. 1054.7 ± 20.4 pg/mL and 1.7±0.2 vs.
9.4±1.1 nmol/mg protein, p<0.05, respectively). The mean level of TNF-α, CINC-1and gastric MDA in Aloe veratreated
group were improved as compared with NSAIDs group (85.1±49.1 vs. 35.2±1.6 pg/mL, 1054.7 ± 20.4 vs.
813.6±239 pg/mL and 9.4±1.1 vs. 2.7±0.6 nmol/mg protein, p < 0.05, respectively). Five rats in NSAIDs group
developed moderate to severe gastric inflammation and erosions. The gastric erosions and neutrophil infiltration
scores were significantly reduced in Aloe vera treated group.
Conclusion: Aloe vera attenuated NSAIDs induced gastropathy in rats by the reduction of oxidative
stress, inflammatory cytokines, and improvement of gastric injury.
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file/Thai-Journal-of-gastroenterology-vol-14-no-2-4197224.pdf