Abstract :
Background: A specific mutation in the p53 gene at codon 249 has been detected in HCC from areas
with high exposure to dietary contaminated with aflatoxin B1 (AFB1) , whereas this mutation is absent from HCC
in regions with negligible exposure to AFB1.
Objective: The aim of our study was to examine p53 codon 249 mutation in HCC tissue from patients
resided in Northern Thailand, and analyzed the relationship between this mutation and clinicopathologic factors.
Patients and Methods: Between January 2001 to June 2003, total 25 liver samples were collected
from HCC patients. Mutation of the p53 gene at codon 249 was detected by SOMA.
Results: Of 25 cases, there were 21 samples (84%) with p53 gene expression, and 4 samples were
undetectable (16%). Mutation at codon 249 of p53 gene was detected in 6 samples (24%), and wild-type p53 was
found in 15 samples (60%). There was no statistically significant association between p53 mutation at codon 249
and age, HBV infection, HCV infection, Child class, tumor size, or AFP level. Only male sex had correlation with
this mutation. (p <0.05)
Conclusion: HCC from Northern Thailand has high prevalence of p53 codon 249 mutation compare to
other countries with high incidence of aflatoxin exposure. Further studies in non HCC patients as well as the
analysis of aflatoxin contamination in food product in Thailand is needed.
[Thai J Gastroenterol 2004; 5(3): 151-156] |
file/Thai-Journal-of-gastroenterology-vol-5-no-3-2353250.pdf